Protein Variants | Comment | Organism |
---|---|---|
H461Y | the mutant shows about 1.2fold increased activity with L-arabinose compared to the wild type enzyme | Escherichia coli |
R358I | the mutant shows about 1.2fold increased activity with L-arabinose compared to the wild type enzyme | Escherichia coli |
R358P | the mutant shows about 1.4fold increased activity with L-arabinose compared to the wild type enzyme | Escherichia coli |
R358S | the mutant shows about 1.4fold increased activity with L-arabinose compared to the wild type enzyme | Escherichia coli |
R520P | the mutant shows about 1.5fold increased activity with L-arabinose compared to the wild type enzyme | Escherichia coli |
R520Y | the mutant shows about 2fold increased activity with L-arabinose compared to the wild type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 6 mM used in assay conditions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | Escherichia coli | - |
D-ribose 5-phosphate + D-xylulose 5-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P27302 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose + lithium beta-hydroxypyruvate | 48% conversion after 24 h | Escherichia coli | L-gluco-heptulose + CO2 + Li+ | - |
? | |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | D-ribose 5-phosphate + D-xylulose 5-phosphate | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Escherichia coli |